Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on osteoblast differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or osteogenic differentiation medium (OM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and matrix mineralization was analyzed by Alizarin Red S staining. Representative light microscopic images of Alizarin Red S staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 versus osteogenic differentiated cells (OM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on the adipogenic differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or adipogenic differentiation medium (AM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and lipid droplet formation was analyzed by Oil Red O (ORO) staining. Representative light microscopic images of ORO staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 and ** p < 0.01 versus adipogenic differentiated cells (AM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Copper tannic acid coordination nanosheet as a potent in-situ antibiotic sustained-release carrier for chronic osteomyelitis

doi: 10.1007/s10856-025-06979-z

Figure Lengend Snippet: The effect of Van@CuTA on BMSCs and HUVECs. A CCK-8 assay demonstrated no toxicity of Van@CuTA toward BMSCs. B Representative images of alizarin red staining, revealing increased calcium deposition in BMSCs treated with Van@CuTA. C , D Representative images and statistical analysis of HUVECs migration assay demonstrating a significant increase in migrated cells following Van@CuTA treatment. E – G Tube formation assay images and corresponding quantifications of total tube length and node number per view indicated that Van@CuTA significantly promoted HUVECs’ tube formation. (* indicated p < 0.05)

Article Snippet: Primary bone marrow mesenchymal stem cells (BMSCs) and Human Umbilical Vein Endothelial Cells (HUVECs) were obtained from ATCC.

Techniques: CCK-8 Assay, Staining, Migration, Tube Formation Assay